ACCUCARETM
ANTI-STREPTOLYSIN (ASO) REAGENT
 Latex test without predilution
Catalog No: Product Description: Pack Size:
ASO 10 ASO Reagent 10 test
ASO 25 ASO Reagent 25 test
ASO 50 ASO Reagent 50 test

ANTISTREPTOLYSIN (ASO) REAGENT SET
For the qualitative and quantative measurement of antibodies to streptococcal exoenzymes in human serum.

INTRODUCTION
 The group A b-hemolytic streptococci produces various toxins that can act as antigens. One of these exotoxins streptolysin-O, was discovered by Todd in 1932. A person infected with group A b-hemolytic streptococci produces specific antibodies against these exotoxins, one of which is antistreptolysin-O. The quantity of this antibody in a patient's serum will establish the degree of infection due to the b-hemolytic streptococci. The usual procedure for the determination of the antistreptolysin titreis based on the inhibitory effect that the patient's serum produces on the hemolytic power of a pretitrated and reduced streptolysin-O. However, the antigen-antibody reaction occurs independently of the hemolytic activity of streptolysin-O. This property enables the establishment of a qualitative and quantitative test for the determination of the antistreptolysin-O by agglutination of latex particles on slide.

PRINCIPLE
 The ASO reagent contains Latex particles coated with streptolysin o antigen. When the reagent is mixed with serum containing ASO at a level greater than 200 IU/ml the particles will agglutinate.

TEST SENSITIVITY
 200 IU/ml
 SAMPLE
 Serum

REFERENCE VALUES
Adults < 200 IU/ml

REAGENTS
 Reagent 1 : ASO Latex Reagent
Reagent 2 : Positive Control Sera
Reagent 3 : Negative Control Sera

ACCESSORIES
 Slides, Stirrer rods, Droppers

STABILITY: All reagents and control are ready for use and stable upto the expiry date when stored at 2-8°C

QUALITATIVE DETERMINATION
 Add in different circles of the slides: Serum to be tested 1 drop (50 ml) Positive Control 1 drop (50 ml) Negative Control 1 drop (50 ml) In all circles add: ASO Latex reagent 1 drop (50 ml) Mix and spread with the stirring rod to fill the test circle. Rotate the slide and observe for any agglutination which should occur within two minutes.

INTERPRETATION OF THE RESULTS
 Marked agglutination indicates an ASO concentration above 200 IU/ml. All the positive samples should be tested by a semi quantitative method.

SEMI-QUANTATIVE DETERMINATION
Prepare sample dilutions with saline 1:2, 1:4,1:8,1:16 etc. Test each dilution according to the qualitative procedure until no further agglutination is observed. The ASO concentration can then be estimated from the last dilution with the visible agglutination.

ASO (IU/ml) = highest dilution with positive reaction x reagent sensitivity (200 IU/ml).

REFERENCES
 Plotz and Singer, Am. S. Med.22, (1979).
Adams, L.E., Hass, E.J.Amer. Technical 48 (1978).
Ditto, W. Am. Soc. Clin. Pat. 69, (1976).
Mormauselle, D. Immunochemistry 9, (1972).
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