ACCUCARETM
ACID PHOSPHATASE
Kinetic color method
Order Information
Catalog No: Product Description: Pack Size:
ACP 10 ACID PHOSPHATASE 10 X 1.1ML
ACP 30 ACID PHOSPHATASE 10 X 3 ML

METHOD :
Kinetic color test

PRINCIPLE
In acid environment, alpha-naphthyl phosphate is hydrolysed by acid phosphatase to produce alpha-napthol and phospate. Alpha-napthol reacts with diazo-2-chloro-5-toluene (Fast Red TR Salt) forming an azo dye compound which absorbs maximally at 405 nm and is directly proportional to total acid phosphatase activity. When the activity is measured in the presence of tartarate, the prostatic activity is inhibited. The difference between Total (ACPT) and Non-prostatic acid phosphatase (ACPNP) corresponds to the prostatic fraction.

REAGENTS
Reagent I : Buffer reagent
Reagent II : Substrate reagent
Reagent III : Tartarate reagent
Reagent IV: Acetate reagent

SAMPLE
Serum. Do not use plasma.
Use fresh serum or Acidify 1ml of serum with 20 µl Acetate reagent & store at 2 - 8°C till further use.

REFERENCE VALUES
37°C Male Female
Total ACP 0-11 IU/L 0-4 IU/L
Prostatic ACP 0-7.3 IU/L  

The reference values are to be considered as indicative only. Every laboratory should establish its own normal range.

QUALITY CONTROL :
Accutestrol N - H

REFERENCES
Bohuen, C. Clin. Chem. Acta 16, 155 1957).
Mann, C. Land yoe, JH., Anal. Chem. 28, 202 (1955).


REAGENT PREPARATION
(A) Total acid phosphatase:
Dissolve the contents of one vial of Substrate with the
volume of buffer as specified on the vial & label it as 'A'.
(B) Non-prostatic acid phosphatase:
Dissolve the contents of one vial of Substrate with the
volume of buffer as specified on the vial & label it as B,
then add 10 ul of Sodium Tartarate in 1ml of reconstituted
reagent.

STABILITY : 7 days at 2-8° C/6 hrs at R.T.

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1 : 10
Delay/Lag/time 300 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit <0.4
Factor 743
Low Normal Refer reference values
High Normal Refer reference values
Linearity 75 IU/L

MANUAL PROCEDURE
PIPETTE directly into tubes A or B 1 ml of reagent

Then add
Sample 0.1 ml

Mix well and after 5 min 37°C read absorbance values and start timer simultaneously. Measure absorbance increase every minute during 3 minutes p A / min)

CALCULATION AND LINEARITY
p A/min. x 743 = U/l total Acid Phosphatase
p A/min. x 743 = U/l non-prostatic Acid Phosphatase

The method is linear upto a concentration of 75 IU/L
Lab Care Diagnostics (India) Pvt. Ltd.
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