ACCUCARE®
ALBUMIN REAGENT
B.C.G. colorimetric test
Order Information
Catalog No: Product Description: Pack Size:
ALB 100 ALBUMIN 2 X 50 ML
ALB 200 ALBUMIN 2 x 100 ml

METHOD
Colorimetric end point test

PRINCIPLE
 The measurement of serum albumin is based on its quantitative binding to the indicator 3, 3', 5, 5' - tetrabo-m-cresol sulphophthalein (bromocresol green, BCG). The albumin-BCG complex absorbs maximally at 578 nm.

SAMPLE
 Serum or Plasma

REFERENCE VALUES
Serum/ Plasma
1.8 g / dl - 5.1 g / dl

QUALITY CONTROL
 Accutestrol N - H

REAGENTS
Reagent I : BCG Reagent
Reagent II : Albumin standard : 4 g/dl (store at 2 - 8°C)

REFERENCES
 E.M. Gindler and J.O. Westgard Clin. Chem., (1973), 6,4. J.O. Westgard, M.A. Poquette, Clin. Chem., (1973) 19, 647.
 REAGENT PREPARATION
The reagent is ready to use.

STABILITY :
Upto expiry date when stored at room temperature.

AUTOMATED PARAMETERS
Wave length 620 nm
Cuvette 1 cm light path
Reaction Temperature 20 - 25°C
Measurement Against reagent blank
Reaction End point

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
SAMPLE     5 µl
STANDARD   5 µl  
REAGENT 1000 µl 1000 µl 1000 µl

Mix well and wait for 5 min at 20 - 25°C. Measure the absorbance of the sample (Ac) and standard (As) against reagent blank.

CALCULATION AND LINEARITY
Ac/As x Conc. Std. = g/dl Albumin

This method is linear upto a concentration of 8.0 g/dl.
If the concentration exceeds this value, the sample should be diluted 1:1 with 0.9% saline solution and reassayed. Multiply the result by 2.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 554 2109/ 00 91 22 554 1558 Fax: 00 91 22 554 3541
E-mail : accucare@labcarediagnostics.com