ACCUCARETM
ALKALINE PHOSPHATASE
Kinetic test optimized according to IFCC recommendations
Order Information
Catalog No: Product Description: Pack Size:
ALP 50 ALKALINE PHOSPHATASE 5 X 10 ML
ALP 100 ALKALINE PHOSPHATASE 5 X 20 ML
ALP 200 ALKALINE PHOSPHATASE 4 X 50 ML

METHOD
Kinetic UV Test

PRINCIPLE
p-Nitrophenyl Phosphate is converted to p-Nitrophenol and Phosphate by alkaline Phosphatase. The increase of absorption at 405 nm is proportional to the alkaline Phosphatase concentration in the sample.

REAGENTS
Reagent I : Buffer Reagent
Reagent II : Substrate reagent

SAMPLE: Serum or plasma.

REFERENCE VALUES
  ADULTS
37°C 25 - 147 U/L

The reference values are to be considered as indicative only. Every Laboratory should establish is own normal ranges.

QUALITY CONTROL
Accutestrol N - H

REFERENCES
Fundamental of Clinical Chemistry, Young D.S, Teitz, N. Fundamentals of Clinical Chemistry 602/609, Kaplan, M.M. New England.

REAGENT PREPARATION
Dissolve the contents of one vial of substrate reagent with the volume of buffer reagent as specified on the substrate vial. 		STABILITY: 21 days at 2 - 8°C.

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1 : 50
Delay/Lag/time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit <0.85
Factor 2720
Low Normal 25 U/L
High Normal 147 U/L
Linearity at 37°C 800 U/L

MANUAL PROCEDURE

 PIPETTE INTO TEST TUBE
Sample 20 µl
Reagent 1000 µl

Mix well and Incubate at 37°C for 60 Secs. Measure absorbance increase every 30 Secs for 2 minutes and determine the p A/min.

CALCULATION AND LINEARITY
p A/min. x 2720 = U/l Alkaline Phosphatase

The method is Linear upto a concentration of 800 U/L.
Lab Care Diagnostics (India) Pvt. Ltd.
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