ACCUCARETM
ALKALINE PHOSPHATASE - SLR
Kinetic test optimized according to IFCC recommendations
Order Information
Catalog No: Product Description: Pack Size:
ALPSLR 25 ALK- PHOSPHATASE
 1 x 25 ml
ALPSLR 125 ALK- PHOSPHATASE 5 x 25 ml
ALPSLR 200 ALK- PHOSPHATASE 4 x 50 ml

METHOD
Kinetic UV Test


PRINCIPLE
p-Nitrophenyl Phosphate is converted to p-Nitrophenol and Phosphate by alkaline Phosphatase. The increase of absorption at 405 nm is proportional to the alkaline Phosphatase concentration in the sample.


REAGENTS
Reagent I : Buffer Reagent
Reagent II : Substrate reagent


SAMPLE: Serum or plasma.

REFERENCE VALUES
  ADULTS
37°C 25 - 147 U/L


The reference values are to be considered as indicative only. Every Laboratory should establish is own normal ranges.


QUALITY CONTROL
Accutestrol N - H


REFERENCES
Fundamental of Clinical Chemistry, Young D.S, Teitz, N. Fundamentals of Clinical Chemistry 602/609, Kaplan, M.M. New England.		 REAGENT PREPARATION
Mix 4 ml of Buffer reagent with 1 ml of substrate reagent.

STABILITY : The working reagent is stable for 4 weeks at
2 - 8°C

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1 : 50
Delay/Lag/time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit <0.85
Factor 2720
Low Normal 25 U/L
High Normal 147 U/L
Linearity at 37°C 2000 U/L

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
Sample 20 µl
Reagent 1000 µl

Mix well and Incubate at 37°C for 60 Secs. Measure absorbance increase every 30 Secs for 2 minutes and determine the p A/min.

CALCULATION AND LINEARITY

p A/min. x 2720 = U/l Alkaline Phosphatase

The method is Linear upto a concentration of 2000 U/L.
Lab Care Diagnostics (India) Pvt. Ltd.
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