ACCUCARETM
CHOLINESTERASE
Butyrylthiocoline potassium hexacyanoferrate (III) method
Order Information
Catalog No: Product Description: Pack Size:
CHOLEN 25 CHOLINESTERASE 1 x 25 ml

METHOD
Kinetic test

PRINCIPLE
Butyrylthiocoline is hydrolysed by cholinesterase to produce thiocoline in the presence of potassium hexacyanoferrate (III), the absorbance decrease is proportional to the cholinesterase activity of the sample.

SAMPLE:

Serum
Heparinised plasma or EDTA plasma

REFERENCE VALUES
At 37°C
4850-12000 U/l

The reference values are to be considered as indicative only. Every laboratory should establish its own normal range.

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I : Buffer reagent
Reagent II : Butyrylthiocoline iodide Reagent

REFERENCES
Knedel, B., Boettger R., klin. Wschr., (1967), 45, 325. Arbeitsgrupee enzyme Chemie (1989) Mitt Dtsch Ges Klin Cheni PS20PS, 123-124.

REAGENT PREPARATION
Mix 4 parts of Reagent I with 1 part of Reagent II.

WORKING REAGENT STABILITY: 30 Days at 2-8°C

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Temperature 37°C
Measurement Against Water
Sample/Reagent 1: 67
Reaction Kinetic
Reaction Direction Decreasing
Delay/Lag/time 60 Secs
Interval time 30 Secs
No of Readings 03
Blank Absorbance Limit Factor 62000
Low Normal at 37°C 4850 U/l
High Normal at 37°C 12000 U/l
Linearity at 37°C 12000 U/l

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE

SAMPLE 15 µl
REAGENT 1000 µl

Mix well and wait for 1 minute. Measure absorbance decrease after 30, 60 and 90 seconds. Determine the p A/minute.

CALCULATION AND LINEARITY
p A/minute x 62000 = U/l cholinesterase

The method is linear up to 12000 U/l
Lab Care Diagnostics (India) Pvt. Ltd.
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