ACCUCARETM
C-REACTIVE PROTEIN (CRP) REAGENT
Latex test without predilution
Catalog No: Product Description: Pack Size:
CRF 10 CRF Reagent 10 test
CRF 25 CRF Reagent 25 test
CRF 50 CRF Reagent 50 test

C-REACTIVE PROTEIN (CRP) LATEX REAGENT SET
For the qualitative and quantative measurement of C-Reactive protein (CRP) in human serum

INTRODUCTION
C-reactive protein (CRP), the classic acute -phase of human serum, is synthesized by hepatocytes. Normally, it is present only in trace amounts in serum, but it can increase by as much as 1000-fold in response to injury or infection. The clinical measurement of CRP in serum therefore appears to be a valuable screening test for organic disease and a sensitive index of disease activity in inflammatory, infective and ischemic conditions. MacLeod and Avery found that antibody produced against purified CRP provided a more sensitive test than the C-polysaccharide assay. Since that time a number of immunological assay have been devised to measure CRP such as capillary precipitation, double immunodiffussion and radical immunodiffusion. The CRP reagent kit is based on the principal of the latex agglutination assay described by Singer and Plotz. The major advantage of this method is rapid two (2) minute reaction time.

PRINCIPLE
The CRP reagent contains Latex particles coated with Anti-Human CRP antibody. When the reagent is mixed with serum containing CRP at a level greater than 0.6 mg/dl the particles will agglutinate.

SENSITIVITY AND RANGE The sensitivity is of 0.6 mg/dl of C-reactive protein according to the World Health Organisation (WHO) international Reference preparation.

SAMPLE
Serum

REFERENCE VALUES
< 0.6 mg/dl		 REAGENTS
Reagent 1 : CRP Latex Reagent
Reagent 2 : Positive Control Sera
Reagent 3 : Negative Control Sera

ACCESSORIES
Slides, Stirrer rods, Droppers

STABILITY: All reagents and control are ready for use and stable upto the expiry date when stored at 2-8°C

QUALITATIVE DETERMINATION

Add in different circles of the slides :
Serum to be tested 1 drop (40 µl)
Positive Control 1 drop (40 µl)
Negative Control 1 drop (40 µl)
In all circles add:  
ASO Latex reagent 1 drop (40 µl)

Mix and spread with the stirring rod to fill the test circle. Rotate the slide and observe for any aggtlutination which should occur within two minutes.

INTERPRETATION OF THE RESULTS
All the positive samples should be tested by a semi quantitative method.

SEMI-QUANTATIVE DETERMINATION
Prepare sample dilutions with saline 1:2, 1:4, ….1:64. Test each dilution according to the qualitative procedure until no further agglutination is observed. The CRP concentration can then be estimated from the last dilution with the visible agglutination.

CRP (mg/dl) = highest dilution with positive reaction x reagent sensitivity (0.6 mg/dl).

REFERENCES
Singer.J.M., Piotz, C.M., Parker, E. & Elster, S.F. Am.J. Clin. Path. 28 (1975) 611.- Nilsson, L.A., Acta Path Microb Scand., 73 (68) 129.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com