SUMMARY AND EXPLANATION
The reagent test areas of urine reagent strips are ready to use upon removal from the bottle. The entire reagent strip is disposable. No additional laboratory equipment is necessary for testing. The directions must be followed exactly. Accurate timing is essential to provide optimal results. The reagent strips must be kept in the bottle with the cap tightly closed (as specified on the cap) to maintain reagent reactivity. To obtain optimal reasons, it is necessary to use fresh, well mixed, and uncentrifuged urine.

TEST PRINCIPLES :
pH: This test is based on a double indicator principle that gives a broad range of colors covering the entire urinary pH range. Colors range from orange through yellow and green to blue.

Ketone: 7.1% w/w sodium nitroprusside balanced with buffer and non reactive ingredients.

WARNINGS AND PRECAUTIONS
Urine reagent strips are for in vitro diagnostic use.

STORAGE
Store at temperature between 4-30ºC (39-86ºF) and out of direct sunlight. Do not use after expiration date.

RECOMMENDED HANDLING PROCEDURES
All unused strips must remain in the original bottle. Transfer to any other container may cause reagent strips to deteriorate and become unreactive. Do not remove desiccant(s) from bottle.

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Glucose Test : This reagent test area may be read at 10 seconds for qualitative results or 30 seconds for quantitative results. The test is specific for glucose: no substance excreted in urine other than glucose is known to give a positive result. The reagent area does not react with lactose, galactose, fructose, non reducing metabolites of drugs; e.g. salicylates and nalidixic acid. This test may be used to determine whether the reducing substance found in urine is glucose. Approximately 0.1g of glucose per dl or urine is detectable.

Ketone Test: The ketone test area provides semi-quantitative results (small, moderate, and large) and reacts with acetoacetic acid in urine. It does not react with beta-hydroxybutyric acid or acetone. The reagent area detects as little as 5 to 10 mg acetoacetic acid per dl of urine.

Protein: This test is based on the protein error-of-indicators principle. At a constant pH, the development of any green color is due to the presence of protein. Colors range from yellow for "Negative" through yellow-green and green-blue for "Positive" reactions.

Glucose: This test is based on a double sequential enzyme reaction. One enzyme, glucose oxidase, catalyzes the reaction of hydrogen peroxide with a potassium iodide chromogen to oxidize the chromogen to colors ranging from green to brown.

Ketone: This test is based on the reaction between acetoacetic acid with nitroprusside. The colors range from buff-pink, for a "Negative" reading to purple.

REAGENTS
(Based on dry weight at time of impregnation)

pH: 0.2% w/w methyl red; 2.8% w/w bromthymol blue; 97% w/w non reactive ingredients.

Protein: 0.3% w/w tetrabromphenol blue; 99.7% w/w buffer and non reactive ingredients.

Glucose: 16.3% w/w glucose oxidase (1.3 IU); 0.6% peroxidase (3300 IU); 7.0% w/w of potassium iodide; 76% w/w buffer and non reactive ingredients.

PROCEDURES: MUST BE FOLLOWED EXACTLY TO ACHIEVE RELIABLE TEST RESULTS
1. Remove one strip from bottle and close the cap immediately. Completely immerse reagent areas of the strip in FRESH urine and remove immediately to avoid dissolving out reagents.
2. While removing, run the edge of the strip against the rim of the urine container to remove excess urine. Hold the strip in a horizontal position to prevent possible mixing of chemicals from adjacent reagent areas and/or soiling of hands with urine.
3. Compare reagent areas to corresponding color chart on the bottle label at the time specified. HOLD STRIP CLOSE TO COLOR BLOCKS AND MATCH CAREFULLY.