ACCUCARETM
CHOLESTEROL HDL
Precipitant Phosphotungstic Acid Reagent
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
LDHSLR 10 LDH(L-P) 1 X 10 ml

Method
Kinetic Test

Principle
Lactate is oxidized to Pyruvate in the presence of NAD by the action of lactate dehydrogenase. The rate of formation of NADH is directly proportional to LDH concentration.

Reagents
Reagents I : Buffer reagent
Reagents II : Enzyme reagent

Sample
Serum free of hemolysis

REFERENCE VALUES
Males 50-166 U/L 30ºC
  80-285 U/L 37ºC
Females 60-132 U/L 30ºC
  103-227 U/L 37ºC

Quality Control
Accutestrol N - H References
Teitz, R.W. Henry, R.J. Young, D.S. 123.

Reagent Preparation
Mix 9 ml of Buffer reagent with 1ml of Enzyme reagent

STABILITY: The working reagent is stable for 30 days at
2-8º C

AUTOMATED PARAMETERS
Wave length 340mm
Cuvette 1 cm light path
Reaction Type 37º C
Measurement Against distilled water
Reaction Type Kinetic Test
Reaction Direction Increasing
Delay/Lag/Time 60 secs
Interval Time 30 secs
No. of Readings 04
Blank Absorbance Limit <
Factor 6592
Low Normal at 37º C 80 U/L
High Normal at 37º C 285 U/L
Linearity at 37ºC 1200 U/L

Manual Procedure

Pipette into test tubes
Working Reagents 1000 ml (make it micro lit)
Sample 25 ml (make it micro lit)

Calculation and linearity
340 nm : Activity (U/L) = D A/min x 6592

Linearity
at 37º C is 1200 U/L
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com