Order
Information
| Catalog No: |
Product Description: |
Pack Size: |
| MAG 25 |
MAGNESIUM |
25 x 1 ml |
METHOD
Colorimetric Endpoint Test
PRINCIPLE
At alkaline pH magnesium reacts with xylidyl blue and produces
a chelating red coloured compound. The red increasing or the blue
decreasing colors are proportional to magnesium concentration.
SAMPLE
It is recommended to use serum.
When using plasma avoid EDTA which may increase results. Urine should
be previously acidified to acid pH value (pH 3-4) by adding some
drops of HCL. Then dilute 1:5 with distilled water.
REFERENCE VALUES
| SERUM
|
1.9
- 2.5 mg/dl |
1.6
- 2.0 mEq/l |
| CSF |
2.4
- 3.1 mg/dl |
1.9
- 2.5 mEq/l |
| URINE
|
75
- 125 mg/24h |
60-100
mEq/24h |
The reference values are to be considered as indicative only. Every
laboratory should establish its own normal range.
QUALITY CONTROL :
Accutestrol N - H
REAGENTS
Reagent I : Xylidyl Blue Reagent
Magnesium Std. : 5 mg/dl
REFERENCES
Bohuen, C. Clin. Chem. Acta 16, 155 1957).
Mann, C. Land yoe, JH., Anal. Chem. 28, 202 (1955).
Fragay DA., Casey, Clin, Biochem., 791, (1974).
|
REAGENT PREPARATION
All reagents are ready to use.
STABILITY: Stable upto expiry date at Room Temperature
AUTOMATED PARAMETERS
| Wave length |
520 nm |
| Cuvette |
1 cm light path |
| Temperature |
Room Temperature |
| Measurement |
Against Reagent Blank |
| Sample/Reagent |
1: 100 |
| Reaction Type |
End point |
| Incubation |
5 mins. |
| Low Normal |
1.9 mg/dl |
| High Normal |
2.5 mg/dl |
| Linearity |
10.0 mg/dl |
MANUAL PROCEDURE
PIPETTE INTO TEST TUBE
| |
BLANK |
STD |
SAMPLE |
| DI
WATER |
10µl |
- |
- |
| STANDARD |
- |
10µl |
- |
| SAMPLE |
- |
- |
10µl |
| REAGENT
|
1000µl |
1000µl |
1000µl |
Mix well and wait for 5 minutes at room temperature. Measure final
absorbance of the sample (Ac) and standard (As) against reagent
blank.
CALCULATION AND LINEARITY
| Ac / As x Conc. Std. = mg / dl Serum |
| Ac / As x Conc. Std. = mg / dl Urine |
The standard concentration is mentioned on the vial.
This method is linear to 10 mg/dl.
NOTES :
Metallic ions and proteins do not interfere with the assay.
Use disposable plasticware to run tests.
|
