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ACCUCARETM
RHEUMATOID FACTOR (RF) REAGENT Latex test without predilution |
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RHEUMATOID FACTOR (RF) REAGENT SET For the qualitative and quantative measurement of RF in human serum INTRODUCTION Rheumatoid factors (RF) are antibodies directed against antigenic sites in the Fc fragment of human and animal IgG. Their frequent occurrence in rheumatoid arthritis makes them useful for diagnosis and monitoring of the disease. One method used for rheumatoid factor detection is based on the ability of rheumatoid arthritis sera to agglutinate sensitized sheep red cells, as observed by waaler and Rose. A more sensitive reagent consisting of biologically inert latex beads coated with human gamma globulin was later described by Singer and Plotz. The RF kit is based on the principle of latex agglutination assay of Singer and Plotz. The major advantage of this method is rapid performance (2 minute reaction time) and lack of heterophile antibody interference. PRINCIPLE The R.F. reagent contains Latex particles coated with human gamma globulin. When the reagent is mixed with serum containing RF at a level greater than 8.0 IU/ml the particles will agglutinate. TEST SENSITIVITY The sensitivity is of 8 IU/ml of rheumatoid factor according to the World Health Organisation (WHO) International Reference preparation. SAMPLE Serum REFERENCE VALUES Adults < 8 IU/ml |
REAGENTS Reagent 1 : RF Latex Reagent Reagent 2 : Positive Control Sera Reagent 3 : Negative Control Sera ACCESSORIES Slides, Stirrer rods, Droppers STABILITY: All reagents and control are ready for use and stable upto the expiry date when stored at 2-8°C QUALITATIVE DETERMINATION Add in different circles of the slides :
In all circles add : RF Latex reagent 1 drop (40 µl) Mix and spread with the stirring rod to fill the test circle. Rotate the slide and observe for any agglutination which should occur within two minutes. INTERPRETATION OF THE RESULTS Marked agglutination indicates an RF concentration above 8 IU/ml. All the positive samples should be tested by a semi quantitative method. SEMI-QUANTATIVE DETERMINATION Prepare sample dilutions with saline 1:2, 1:4,1:64. Test each dilution according to the qualitative procedure until no further agglutination is observed. The RF concentration can then be estimated from the last dilution with the visible agglutination. RF (IU/ml) = Highest dilution with positive reaction x reagent sensitivity (8 IU/ml). REFERENCES Waaler.E., Acta Path. Micr. Scand., 17,1 & 2 (40). Bandila, K.L., & Mc Duffie, F.C., Arthritis Rheum. 12 (1969) 74. |
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Lab
Care Diagnostics (India) Pvt. Ltd. 16-A, 'A' Wing , 1st Floor, Sita Estate, Mahul Road, Aziz Baug, Chembur, Mumbai 400 074. India Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541 E-mail : accucare@labcarediagnostics.com |