ACCUCARETM
ALPHA-AMYLASE
Kinetic colorimetric test
Order Information
Catalog No: Product Description: Pack Size:
AMY SLR 10 ALPHA-AMYLASE 1 X 10 ml


METHOD
Kinetic colorimetric test


PRINCIPLE
2-chloro-4-nitrophenyl-alpha-D-maltotrioside (CNPG3) is hydrolyzed by alpha-amylase to produce 2-chloro-4-nitrophenol (CNP). The amount of 2-chloro-4-nitrophenol, monitored at 405 nm, is directly proporational to alpha-amylase activity.


SAMPLE:
Serum, heparinized plasma urine diluted 1:2.


STABILITY
serum upto 7 days at R.T. 3 months at 2-8°C
Urine : 2 days at R.T. 10 days at 2-8°C


REFERENCE VALUES
Serum : Upto - 130 U/l
Urine : Upto - 490 U/l


QUALITY CONTROL
Accutestrol N - H


REAGENTS
Reagents I : CNPG3 Substrate reagent


REFERENCES
Ranson, JHC. Curr Prob Surg 1979; 16:1.
Salt WB II, Schnkers S. Medicine 1976; 55:269.
Stefanini P, Ermini M, J Am Surg 1965; 110:866
Henry RJ, Chiamori N. Clin. Chem 19610; 6:434.
Kaufman RA, Teitz NW. Clin. Chem 1980; 26:486.		 REAGENT PREPARATION
The reagent is ready to use.

STABILITY : Upto expiry date when stored at 2-8°C

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1:40
Delay/Lag/ time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank absorbance limit <0.7
Factor 3178
Low Normal at 37°C 0 U/L
High Normal at 37°C 130 U/L
Linearity 2000 U/L

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
SAMPLE 25 µl
REAGENT 1000 µl

Mix well and let stand for 1 min. read initial absorbance and start timer simultaneously. Measure absorbance increase every minute for 3 minutes (p A/min).

CALCULATION AND LINEARITY
p A/min x 3178 = U/l SERUM.
p A/min x 9534 = U/l URINE

This method is linear upto a concentration of 2000 U/l
sample above this concentration should be diluted 1:2.
Multiply the result by 2.

NOTES Avoid contamination of reagent, samples and glassware by saliva because it has a high amylase content. Do not pipette by mouth. Avoid Haemolysis.
Avoid anticoagulants as EDTA or sodium citrate.
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