ACCUCARETM
BUN UV-SLR
(Kinetic test UV - F.T.)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
BUNSLR 125 BUN UV-SLR 5 x 25 ml
BUNSLR 200 BUN UV-SLR 4 x 50 ml


METHOD
Kinetic UV method


PRINCIPLE
Urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia combines with alpha-oxoglutarate and NADH in the presence of glutamate-dehydrogenase to yield glutamate and NAD.


SAMPLE:
Serum or plasma, urine dilute 1:20.
Dilute urine 1+20 with distilled water. Multiply the result by 21. Stability in the serum and in the plasma : 3 days at +4°C.


REFERENCE VALUES
Serum plasma 7-23 mg/d
Urine 9-16 g/24h


QUALITY CONTROL :
Accutestrol N - H


REAGENTS
Reagent I : Buffer reagent
Reagent II : Enzyme reagent
BUN Std : 20 mg/ dl


REFERENCES
Teitz.N.W; Fundamentals of clinical chemistry, Philadelphia, W.B.Saunders & Co,. Philadelphia, p991 (1976)., Talke H. Schubert GE, Klin Wchers., (1965), 43, 174.

REAGENT PREPARATION
 Mix 4 ml of Buffer reagent with 1 ml of Enzyme reagent.
 STABILITY:The working reagent is stable for 30 days
at 2-8°C

AUTOMATED PARAMETERS
Wave length 340 NM
Measurement Against distilled water
Reaction Type Fixed time kinetic
Cuvette 1 cm light path
Reaction Temperature 37°C
Reaction Direction Decreasing
Sample/Reagent Ratio 1: 100
Delay/Lag/ time 30 Secs
Interval time 60 Secs
No: of Readings 01
Minimum Blank Absorbance > 0.8
Low Normal at 37°C 7 mg/dl
High Normal at 37°C 23 mg/dl
Linearity at 37°C 300 mg/dl

MANUAL PROCEDURE

 PIPETTE INTO TEST TUBE
  STD SAMPLE
SAMPLE   10 µl
STANDARD 10 µl  
REAGENT 1000 µl 1000 µl

Mix well and read after 30 secs initial absorbance of the sample (A1s) and standard (A1std) and start timer simultaneously. Read again, after 60 secs.

CALCULATION AND LINEARITY
(A2s-A1s) / (A2std-A1std) x 20 = mg/dl BUN

The method is linear to a concentration of 300 mg/dl. Dilute samples above this concentration 1+1with 0.9% Nacl solution and repeat assay. Multily the result by 2.

NOTE :
All anticoagulants except ammonia-heparin can be used.

Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com