ACCUCARETM
CALCIUM
Arsenazo - III
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
CARZM 50 CALCIUM 50 X 1 ML
CARZ 100 CALCIUM 2 X 50 ML
CARZ 200 CALCIUM 2 X 100 ML


METHOD
Colorimetric end point test

PRINCIPLE
At a neutral pH, the Ca2+ forms with arsenazo III a complex, the color intensity of which is directly proportional to the concentration of calcium in the sample.

SAMPLE
Serum, heparinised plasma, 24 urine diluted 1:3 with distilled water (adjust pH 3-4 with 0.1 N HCL).

REFERENCE VALUES
SERUM 8.8 - 10.2 mg/dl

The reference values are to be considered as indicative only. Every laboratory should establish its own normal ranges..

QUALITY CONTROL
Accutestrol N - H

REAGENTS
Reagent I : Arsenazo III reagent
Reagent II : Calcium standard : 10 mg/dl

NOTE:
CARZM 50 are specially pre-treated monovials with 1 ML pre-dispensed reagent. Just add 25 µl sample / std. & aspirate. Use the same programme.

REFERENCES
Bishop,M.L.Duben-Von Laufen, J.L,.Burtis, Carl Aa and Ashwood.

 REAGENT PREPARATION
All reagents are ready to use

STABILITY : Upto expiry date when stored at R.T.

AUTOMATED PARAMETERS
Wave length 650 nm
Cuvette 1 cm light path
Measurement Against reagent blank
Reaction Temperature R.T.
Reaction Type End Point
Sample/Reagent Ratio 1:40
Incubation 5 minutes
Blank absorbance limit 0.80
Low Normal 8.8 mg/dl
High Normal 10.2 mg/dl
Linearity 16 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE

  BLANK STD SAMPLE
SAMPLE - - 25 µl
STANDARD - 25 µl -
REAGENT 1000 µl 1000 µl 1000 µl

Mix well, incubate at R.T. 5 mins. Measure final absorbance of
the sample (Ac) and standard (As) against the reagent blank.

CALCULATION AND LINEARITY
Ac/As x C = mg/dl SERUM
Ac/As x C x 3 = mg/dl URINE

C = Concentration Standard

The method is linear to a concentration of 16 mg/dl.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com