ACCUCARETM
CHLORIDE
mercurous (II) thiocyanate method
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
CHLOM 50 CHLORIDE 50 X 1 ML
CHLOM 100 CHLORIDE 2 X 50 ML
CHLOM 200 CHLORIDE 2 X 100 ML

METHOD
Colorimetric endpoint test

PRINCIPLE
Chloride reagent reacts with mercurous thiocyanate to form mercury perchlorate and thiocyanate. Thiocyanate forms a red complex with ferric ions in the presence of nitric acid.

SAMPLE
Serum or heparinised plasma.
Urine diluted 1:2 with distilled water.

REFERENCE VALUES
SERUM 98 - 110 mEq/l
24 h URINE 170 - 250 mEq/l

QUALITY CONTROL
Accutestrol N - H

REAGENTS
Reagent I : Chloride reagent
Reagent II : Chloride standard : 100 mEq / l

NOTE:
CHLOM 50 are specially pre-treated monovials with 1 ML pre-dispensed reagent. Just add 10 µl sample / std. & aspirate. Use the same programme.

REFERENCES
Teitz N.W White, W.L.Mosby, CO St. Louis P. Young. D.S. Henry, R,J,.Chem. (1964), 10, 533.
 REAGENT PREPARATION
The reagent is ready to use.

STABILITY: Upto expiry date when stored at R.T.

AUTOMATED PARAMETERS
Wave length 480 nm
Cuvette 1 cm
Reaction Temperature 20 - 25°C
Measurement Against Reagent blank
Reaction Type End Point
Sample/Reagent Ratio 1: 100
Incubation 5 minutes
Reagent Blank Abs limit <0.5
Low Normal 98 mEq/l
High Normal 110 mEq/l
Linearity 130 mEq/l

MANUAL PROCEDURE PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
SAMPLE - - 10 µl
STANDARD - 10 µl -
REAGENT 1000 µl 1000 µl 1000 µl

Mix well, incubate at 20 - 25°C for 5 min. Measure final absorbance of the sample (Ac) and standard (As) against the reagent blank.

CALCULATION AND LINEARITY
Ac/As x C = mEq/l SERUM or PLASMA
Ac/Ac x C x 2 = mEq/l URINE

C = Concentration Standard .

The method is linear to a concentration of 130 mEq/l.

Lab Care Diagnostics (India) Pvt. Ltd.
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