ACCUCARETM
CK - MB
Measurement of CK-MB activity
(serum and plasma)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
MB 10 CK-MB 10 X 1.1 ML
MB 30 CK-MB 10 X 3 ML

METHOD
Kinetic method

PRINCIPLE
The procedure involves measurement of Creatinine kinase (CK) activity in the presence of an antibody of CK-M monomer. This antibody completely inhibits the activity of CK-MM and CK-BB. Than the CK method is used to quantitatively determine CK-BB activity. CK catalyses the reaction between creatinine Phosphate and ADP, giving creatinine and ATP. The ATP & glucose are converted to ADP & Glucose-6-phosphate by hexokinase. Glucose-6-phosphate dehydrogenase oxidises Glucose-6-phosphate & reduces NAD to NADH. The rate of NADH formation is determined photometrically at 340 nm & is directly proportional to CK-BB activity. The CK-MB activity is obtained by multiplying the CK-BB activity by two.

SAMPLE:
Serum free of hemolysis, heparinised plasma, protected from light. Stability 24 hrs at 2-8°C; 3 days at -20°C

REFERENCE VALUES
0 - 24 U/l (37°C)
0 - 14 U/l (30°C)

QUALITY CONTROL :
Accutestrol N - H

REAGENTS COMPOSITION
Reagent I - Enzyme Reagent
Reagent II - Diluent

REFERENCES
Youngs et. al., Clin. Chem., 21 : 10 (1975)

REAGENT PREPARATION
Dissolve the contents of one vial of enzyme reagent with the volume of diluent as specified on the enzyme vial, swirl to dissolve. 		STABILITY: 14 days at 2-8°C

AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm
Reaction Temperature 37°C
Measurement Against distilled water
Sample/Reagent 1: 20
Reaction Kinetic test
Reaction Direction Increasing
Delay/lag/ time 600 Secs
Interval time 60 Secs
No: of Readings 06
Blank Absorbance limit <0.7
Factor 6752
Low Normal at 37°C 0 U/l
High Normal at 37°C 24 U/l
Linearity 1500 U/l

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
REAGENT 1000 µl

SAMPLE 50µl

Mix well, after 10 min , measure the change in absorbance.
Repeat readings every minute for next 6 minutes.
Calculate p A/min.


CALCULATION AND LINEARITY

p A / min x 3376 = U/l CK-BB
CK-BB x 2 = U/l CK - MB

The method is linear to a concentration of 1500 U/l. Samples above 1500 U/l should be dilute 1:1 with saline, re-assayed and the results multiplied by two.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com