ACCUCARETM
CK - NAC
Kinetic U.V. optimised
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
CK 10 CK-NAC 10 X 1.1 ML
CK 30 CK-NAC 10 X 3 ML

METHOD
Kinetic method

PRINCIPLE
Creatine kinase (CK) catalyses the reaction between creatine phosphate and ADP, giving creatine and ATP. The ATP and glucose are converted to ADP, and glucose-6-phosphate by hexokinase. Glucose-6-phosphate dehydrogenase (G-6-PDH) oxidises glucose-6-phosphate and reduces NAD to NADH. The rate of NADH formation is determined photometrically at 340 nm and is directly proportional to the CK activity in the sample.

SAMPLE:
Serum free of hemolysis, heparinised plasma, protected from light. Stability 24 hrs at 2-8°C; 3 days at -20°C

REFERENCE VALUES
25 - 192 U/l (37°C)
10 - 109 U/l (30°C)

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I - Enzyme Reagent
Reagent I - Diluent

REFERENCES
Youngs et. al., Clin. Chem., 21 : 10 (1975) Moren L.G., Clin. Chem., 23 : 1569 (1977)

REAGENT PREPARATION
Dissolve the contents of one vial of enzyme reagent with the volume of diluent water as specified on the enzyme vial, swirl to dissolve.
STABILITY : 21 days at 2-8°C

AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm
Reaction Temperature 37°C
Measurement Against distilled water
Sample/Reagent 1: 20
Reaction Kinetic test
Reaction Direction Increasing
Delay/lag/ time 120 Secs
Interval time 60 Secs
No: of Readings 02
Blank Absorbance limit <0.7
Factor 6592
Low Normal at 37°C 25 U/l
High Normal at 37°C 192 U/l
Linearity 1500 U/l

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
REAGENT 1000 µl

SAMPLE 25 µl

Mix well, after 2 min , measure the change in absorbance. Read the initial absorbance and start timer simultaneously
Read again after every 60 seconds for 2 minutes. Measure delta A/min.

CALCULATION AND LINEARITY
delta A / min x 6592 = U/l CK

The method is linear to a concentration of 1500 U/l.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com