ACCUCARETM
CREATININE
Without deproteinisation method
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
CRE 100 CREATININE 2 X 50 ML
CRE 200 CREATININE 2 X 100 ML


METHOD Colorimetric kinetic test


PRINCIPLE Creatinine in alkaline solution reacts with picrate to form a coloured complex which absorbs at 500 - 520 nm. The amount of complex formed is directly proportional to the creatinine concentration.


SAMPLE:
Serum, Urine 24h diluted 1 : 20 with distilled water.


Reagent I : Buffer Reagent
Reagent II : Picrate reagent
Reagent III : Creatinine Standard 2 mg/ dl


REFERENCE VALUES
  MEN WOMEN
SERUM 0.8 - 1.4 mg/dl 0.7 - 1.2 mg/dl
24h URINE 1.0 - 2.0 0.8 - 1.8 G/24h


QUALITY CONTROL
Accutestrol N - H


NOTE:
For icteric samples pipette 0.5 ml of buffer reagent into test tube and add 0.1 ml serum sample. Incubate at R.T. for 10 mins. than add 0.5 ml of picrate reagent and aspirate. Rest of the procedure and programme remain the same.


REFERENCES Henry, J.B, Young D.S. teitz N.W, Vasilades, J, Can, Chem (1972), 18. 		REAGENT PREPARATION
Mix equal volumes of solution 1 and 2 and let stand for 30 min.

STABILITY
30 days at 2 - 8°C / 7 days at RT (when stored in a dark bottle.)

AUTOMATED PARAMETERS
Wave length 1 500 nm
Wave length 2 (optional) 630 nm
Measurement Against reagent blank
Cuvette 1 cm light path
Reaction Temperature Room Temperature
Reaction Type Fixed Time
Reaction Direction Increasing
Sample/Reagent Ratio 1 : 10
Delay/Lag/time 30 Secs
Interval time 120 Secs
No: of Readings 01
Low Normal 0.7 mg/dl
High Normal 1.4 mg/dl
Linearity at 37°C 25 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
REAGENT 1 µl BLANK STD Sample
Sample - - 100 µl
Standard - 100 µl -
Reagent 1000 µl 1000 µl 1000 µl

Mix and after 30 secs at R.T., read initial absorbance and start timer simultaneously.
Read again after 2 min. determine p A min. of standard (As) and sample (Ac) against reagent blank.

CALCULATION AND LINEARITY

p Ac/p As x C = mg / dl Creatinine Serum
p Ac/p As x C x 20 = mg / dl Creatinine Urine

The method is Linear upto 25 mg / dl
Lab Care Diagnostics (India) Pvt. Ltd.
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E-mail : accucare@labcarediagnostics.com