ACCUCARETM
GLUCOSE
Enzymatic colorimetric test (GOD-POD)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
GLU 500 GLUCOSE 5 X 100 ML
GLU 2500 GLUCOSE 5 X 500 ML

METHOD
Colorimetric end point test

PRINCIPLE
Glucose is determined after enzymatic oxidation in the presence of glucose oxidase.The hydrogen peroxide formed reacts, under catalysis of peroxidase, with sodium phenolate and 4-aminophenazone to form a red-violet quinoeimine dye as indicator.

REAGENTS
Reagent I : Enzyme reagent
Reagent II : Glucose standard : 100 mg/dl

SAMPLE
Serum, plasma, liquor and urine.
Serum and plasma stability : 7 days at +2 -8°C

REFERENCE VALUES
  Plasma Liquor Urine
Serum 60 - 110 mg/dl 45 - 80 mg/dl <0.5 g/24 h

QUALITY CONTROL
Accutestrol N - H

NOTES
In case of urine, samples should be diluted 1:10 and the
result multiplied by 10.

REFERENCES
Teitz, N.W., Fundamentals of Clin. Chem., Philadelphia. W.B. Saunders (l 970) Trinder. P., "Determination of Blood Glucose Using 4 Aminophenazone." 		REAGENT PREPARATION
Dissolve enzyme reagent with volume of distilled water as
specified on the enzyme vial.
STABILITY : Working Reagent is stable for 365 days at
2 - 8°C. (Store protected from light)

AUTOMATED PARAMETERS
Wave length 505 nm
Reaction Type End Point
Cuvette 1 cm light path
Reaction Temperature 37°C
Reaction Type Increasing
Measurement Against Reagent Blank
Sample/Reagent Ratio 1 : 100
Incubation 15 minutes
Low Normal at 37°C 60 mg/dl
High Normal at 37°C 110 mg/dl
Linearity at 37°C 500 mg/dl

MANUAL PROCEDURE

 PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
Sample - - 10µl
Standard - 10µl -
Reagent 1000µl 1000µl 1000µl

Mix & Incubate for 15 min at 37°C or 30 min at RT. Measure absorbance of Sample (AT) and Standard (AS) against
Reagent Blank. The colour is stable for 30 min at R.T.


CALCULATION AND LINEARITY
Total Glucose (mg/dl) = AT/AS x conc. Standard

The method is linear up to a concentration of 500 mg/dl.
Dilute samples above this concentration 1 : 1 and multiply
the result by
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com