ACCUCARETM
GLUCOSE-SLR
Enzymatic colorimetric test (GOD-POD)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
GLUSLR 500 GLUCOSE - SLR 1 X 500 ML
GLUSLR 1000 GLUCOSE - SLR 1 X 1000 ML


METHOD
Colorimetric end point test


PRINCIPLE
Glucose is determined after enzymatic oxidation in the presence of glucose oxidase. The hydrogen peroxide formed reacts, under catalysis of peroxidase, with phenol and 4-aminophenazone to form a red-violet quinoeimine dye as indicator.


REAGENTS
Reagent I : Enzyme
Glucose Standarad : 100 mg/dl


SAMPLE: Serum, plasma, liquor and urine.
Serum and plasma stability : 7 days at +2-8°C


REFERENCE VALUES
Serum - plasma : 60-110 mg/dl
Liquor 9-16 g/24h
Urine <0.5 g/24 h


QUALITY CONTROL
Accutestrol N - H


REFERENCES
Teitz, N.W., Fundamental of Clin. Chem., Philadelphia. W.B. Saunders (1970)
Trinder. P.,"Determination of Blood Glucose Using 4 Aminophenazone.		 REAGENT PREPARATION
Reagent is ready to use.

STABILITY: Reagent is stable for 365 days at 2 - 8°C.
(Store protected from light)

AUTOMATED PARAMETERS
Wave length 505 nm (490 - 550 nm)
Reaction Type End Point
Cuvette 1 cm light path
Reaction Temperature 37°C
Reaction Type Increasing
Measurement Against reagent blank
Sample/Reagent Ratio 1 : 100
Incubation 15 minutes
Maximum Blank Absorbance 0.50
Low Normal at 37°C 60 mg/dl
High Normal at 37°C 110 mg/dl
Linearity at 37°C 500 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
SAMPLE - - 10µl
STANDARD - 10µl -
REAGENT 1000 µl 1000 µl 1000 µl

Mix & incubate for 15 mins at 37°C or 30 min at RT. Measure
absorbance of sample (AT) and standard (As) against reagent blank.
The color is stable for 30 mins at RT.

CALCULATION AND LINEARITY
Total Glucose (mg/dl) = AT/AS x conc. Standard

This method is linear upto a concentration of 500 mg/dl. Dilute samples
above this concentration 1 : 1 and multiply the result by 2.

Notes
In case of urine, samples should be diluted 1 : 10 and the result multiplied by 10.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com