ACCUCARETM
LDH
(Kinetic test)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
LDH 10 LDH-L 10 X 1.1 ML
LDH 30 LDH-L 10 X 3 ML

METHOD
Kinetic test

PRINCIPLE
Lactate is oxidized to Pyruvate in the presence of NAD by the action of lactate dehydrogenase. The rate of formation of NADH is directly proportional to LDH concentration.

SAMPLE
Serum free of hemolysis

REFERENCE VALUES
Males 50-166 U/L 30°C
80-285 U/L 37°C
Females 60-132 U/L 30°C
103-227 U/L 37°C

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I : Enzyme reagent

REFERENCES
Teitz, R.W. Henry, J.B., Henry, R.J.Young, D.S. 123.

REAGENT PREPARATION
Dissolve contents of each vial of enzyme reagent with the volume of
deionised water as specified on the vial.
STABILITY: 14 days when stored at 2-8°C (protected from light)

AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1: 40
Delay/Lag/time 60 Secs
Interval time 30 Secs
No of Readings 04
Blank absorbance limit <0.6
Factor 6592
Low Normal at 37°C 80 U/L
High Normal at 37°C 285 U/L
Linearity at 37°C 800 U/L

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
Working Reagent 1000 µl
Sample 25 µl

Mix well and after 1 min incubation, measure the change in absorbance
per minute (p A/min.) during 2 minutes.

CALCULATION AND LINEARITY

340 nm : Activity (U/L) = p A/min X 6592

Linearity at 37°C is 800 U/L
Lab Care Diagnostics (India) Pvt. Ltd.
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Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com