ACCUCARETM
CSF / URINE TOTAL PROTEIN
Colorimetric test
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
MTP 100 CSF / URINE TOTAL PROTEIN 2 X 50 ML
MTP 200 CSF / URINE TOTAL PROTEIN 2 X 100 ML

METHOD
Colorimetric

PRINCIPLE
The Total Protein test for Urine and CSF is a dye binding colorimetric method utilising pyrogallol red-molybdate complex, and modified to equalize the reactivities of albumin and gamma - globulin, and provide good precision and linearity. The pyrogallol red dye combines with maximum absorbance at 467 nm. When this complex is combined with protein in acidic conditions, a blue - purple color develops with an increase in absorption at 598 nm.

SAMPLE
Urine samples collected randomly or 24 hr specimens may be used. Store at 2-8°C or freeze the specimens till assayed. CSF should be free of hemolysis. Centrifuge any specimen containing red blood cells or particulate matter. CSF may be stored at 2-8°C for several days until assay.

REFERENCE VALUES
CSF (Lumbar) 8 - 43 mg/dl
Urine 28 - 141 mg/day
Random urine 1 - 14 mg/dl

The reference values are to be considered as indicative only. Every laboratory should establish its own normal ranges.

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I : Pyrogallol red reagent
Protein standard : 100 mg/dl
 REFERENCES Teitz N.W., Textbook of Clinical Chemistry. W.B. Saunders Co. (1986) pp 602-613. Dilena B.A., Penberthy L.A., Fraser C.G. , Clin Chem. (1983) 29 : 356 - 360

REAGENT PREPARATION
The reagents are ready to use

STABILITY: Upto expiry date when stored at 2-8°C.

AUTOMATED PARAMETERS
Wave length 600 nm
Reaction Temperature 37°C
Reaction Type End Point
Cuvette 1 cm light path
Measurement Against reagent blank
Sample/Reagent Ratio 1:50
Incubation 10 minutes
Low Normal (CSF) 8 mg/dl
High Normal (CSF) 43 mg/dl
Linearity 200 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
Sample     20 µl
STANDARD   20 µl  
REAGENT 1000 µl 1000 µl 1000 µl

Mix well, incubate for 10 min at 37°C. Measure absorbance of the sample (Ac) and standard (As) against reagent blank.

CALCULATION AND LINEARITY
Ac/As x Conc. Standard = CSF TOTAL mg/dl
Ac/As x Conc. Standard x Vdl = URINE TOTAL                                              PROTEIN mg/dl

Where Vdl is the urine volume in dl
The method is linear to a concentration of 200 mg/dl.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com