ACCUCARETM
GOT-AST
(Without Pyridoxal Phosphate)
Kinetic UV test - optimised IFCC method
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
GOT 50 SGOT (AST)IFCC 5 X 10 ML
GOT 100 SGOT (AST)IFCC 5 X 20 ML
GOT 200 SGOT (AST)IFCC 4 X 50 ML

METHOD
 Kinetic UV test

PRINCIPLE
 Aspartate transaminase (GOT - AST) catalyses the reaction between alpha - ketoglutaric acid and L-aspartate giving glutamate and oxaloacetate. Oxaloacetate, in the presence of malate dehydrogenase (MDH) reacts with NADH giving malate and NAD. The rate of NADH decrease is determined photometrically and is directly proportional to the GOT activity in the sample.

 REAGENTS
Reagent I : Buffer Reagent
Reagent II : Enzyme reagent

SAMPLE:
Serum or plasma

REFERENCE VALUES
 0 to 35 U/l at 37°C

QUALITY CONTROL
Accutestrol N - H

REAGENT PREPARATION
Dissolve one vial of enzyme reagent with amount of buffer reagent as specified on the enzyme vial.

STABILITY
3 months at 2 - 8°C 		AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Decreasing
Sample/Reagent Ratio 1 : 10
Delay/Lag/time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit >0.8
Factor 1746
Low Normal at 37°C 0 U/L
High Normal at 37°C 35 U/L
Linearity at 37°C 350 U/L

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
SAMPLE 100 µl
REAGENT 1000 µl

Mix well and let stand for 1 min. at 37°C. Measure absorbance decrease per minute during 3 minutes and determine the p A/min

CALCULATION AND LINEARITY
p A/min. x 1746 = U/l AST

The method is Linear upto 350 U/L

NOTES
 Very low initial absorbance suggests very high activity of GOT; dilute the samples appropriately and reassay. Multiply results with dilution factor. Procedure & calculation will be the same.

REFERENCES
Expert Panel on enzyme of the IFCC, Clin. Chem. Acta, 70, PM, (1976), Teitz., N.W.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com