ORDER
INFORMATION
| Catalog No: |
Product Description: |
Pack Size: |
| GOTSLR 25 |
GOT-AST-SLR |
1 x 25 ml |
| GOTSLR 125 |
GOT-AST-SLR |
5 x 25 ml |
| GOTSLR 200 |
GOT-AST-SLR |
4 x 50 ml |
METHOD
Kinetic UV test
PRINCIPLE
Aspartate transaminase (GOT - AST) catalyses the reaction between
alpha - ketoglutaric acid and L-aspartate giving glutamate and oxaloacetate.
Oxaloacetate, in the presence of malate dehydrogenase (MDH) reacts
with NADH giving malate and NAD. The rate of NADH decrease is determined
photometrically and is directly proportional to the GOT activity
in the sample.
REAGENTS
Reagent I : Buffer Reagent
Reagent II : Enzyme reagent
SAMPLE:
Serum or plasma
REFERENCE VALUES
0 to 35 U/l at 37°C
QUALITY CONTROL
Accutestrol N - H
NOTES
Very low initial absorbance suggests very high activity of
GOT; dilute the samples appropriately and reassay. Multiply results
with dilution factor. Procedure & calculation will be the same.
REFERENCES
Expert Panel on enzyme of the IFCC, Clin. Chem. Acta, 70, PM,
(1976), Teitz., N.W.
|
REAGENT PREPARATION
Mix 4 ml of Buffer reagent with 1 ml of Enzyme reagent.
STABILITY: The working reagent is stable for 30 days at
2 - 8°C
AUTOMATED PARAMETERS
| Wave length |
340 nm |
| Cuvette |
1 cm light path |
| Reaction Temperature |
37°C |
| Measurement |
Against distilled water |
| Reaction Type |
Kinetic test |
| Reaction Direction |
Decreasing |
| Sample/Reagent Ratio |
1: 10 |
| Delay/Lag/time |
60 Secs |
| Interval time |
30 Secs |
| No of Readings |
04 |
| Blank absorbance limit |
>0.8 |
| Factor |
1746 |
| Low Normal at 37°C |
0 U/L |
| High Normal at 37°C |
40 U/L |
| Linearity at 37°C |
440 U/L |
MANUAL PROCEDURE
PIPETTE INTO TEST TUBE
| SAMPLE |
100 µl |
| REAGENT |
1000 µl |
Mix well and let stand for 1 min. at 37°C. Measure absorbance
decrease per minute during 3 minutes and determine the
p A/min
CALCULATION AND LINEARITY
| p
A/min. x 1746 = U/l AST |
The method is Linear upto 440 U/L |
