ACCUCARETM
GGT - ALT
Kinetic test UV - optimised method IFCC
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
GGT 10 GAMMA - GT 10 x 1.1 ml
GGT 30 GAMMA - GT 10 x 3 ml

METHOD
 Kinetic test

PRINCIPLE
 Gamma-glutamyl is transferred from gamma-glutamyl-p-nitroanilide to glycylglycine by gamma-GT (Gamma-Glutamyl-Transferase). The p-nitroanine formed absorbs at 405 nm. The amount of p-nitroanine is directly proportional to gamma-GT activity.

SAMPLE
Serum.

REFERENCE VALUES
  WOMEN MEN
37°C 9 - 37 U/l 11 - 43 U/l

The reference values are only indicative in nature. Every laboratory should establish its own normal ranges.

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I : Buffer reagent
Reagent II : Substrate reagent

REFERENCES
 Szasz G., Clin. Chem., (1969), 15: 24,

REAGENT PREPARATION
 Dissolve the contents of one vial of substrate reagent with volume of buffer reagent as specified on the vial.


STABILITY: 21 days at 2-8°C.

AUTOMATED PARAMETERS
Wave length 405 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Increasing
Sample/Reagent Ratio 1 : 10
Delay/Lag/time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit <0.85
Factor 1111
Low Normal at 37°C 9 U/L
High Normal at 37°C 43 U/L
Linearity at 37°C 200 U/L

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
STANDARD 100 µl
REAGENT 1000 µl

Mix well and incubate for 1 min. at 37°C. read initial absorbance and start timer simultaneously; read again after 1,2 and 3 min (p A/min).

CALCULATION AND LINEARITY
p A/min. x 1111 = U/l Gamma GT

The method is linear upto 200 U/L
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com