ACCUCARETM
GPT - ALT
(Without Pyridoxal Phosphate)
Kinetic UV test - optimised IFCC method
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
GPT 50 SGPT(ALT)IFCC 5 X 10 ML
GPT 100 SGPT(ALT)IFCC 5 X 20 ML
GPT 200 SGPT(ALT)IFCC 4 X 50 ML

METHOD
 Kinetic UV test

PRINCIPLE
Glutamic-pyruvic Transaminase (GPT - ALT) catalyses the reaction between alpha - ketoglutaric acid and alanine giving L-glutamic acid and Pyruvic acid. Pyruvic acid, in the presence of lactate dehydrogenase (LDH) reacts with NADH giving lactic acid and NAD. The rate of NADH consumption is determined photometrically and is directly proportional to the GPT activity in the sample.

 SAMPLE
Serum or plasma.

REFERENCE VALUES
0 to 40 U/l
The reference values are only indicative in nature. Every laboratory should establish its own normal ranges.

QUALITY CONTROL
 Accutestrol N - H

REAGENTS
Reagent I : Buffer reagent
Reagent II : Enzyme reagent


REAGENT PREPARATION
Dissolve one vial of enzyme reagent with amount of buffer reagent as specified on the enzyme vial.

STABILITY : 3 months at 2-8°C.
 AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against distilled water
Reaction Type Kinetic test
Reaction Direction Decreasing
Sample/Reagent Ratio 1 : 10
Delay/Lag/time 60 Secs
Interval time 30 Secs
No: of Readings 04
Blank Absorbance limit >0.8
Factor 1746
Low Normal at 37°C 0 U/L
High Normal at 37°C 40 U/L
Linearity at 37°C 350 U/L

MANUAL PROCEDURE

 PIPETTE INTO TEST TUBE
STANDARD 100 µl
REAGENT 1000 µl

Mix well and let stand for 1 min. at 37°C. Measure absorbance decrease per minute during 3 minutes (p A/min)

CALCULATION AND LINEARITY
p A/min. x 1746 = U/l ALT

The method is linear upto 350 U/L

NOTES :
Very low initial extinction suggest very high activity of GPT; dilute the samples appropriately and reassay. Multiply results with dilution factor. Procedure & calculation will be the same

REFERENCES
 Expert Panel on enzyme of the IFCC, Clin. Chem. Acta
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com