ACCUCARETM
TRIGLYCERIDES
Enzymatic colorimetric test - GPO PAP
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
TGL 50 TRIGLYCERIDE 5 X 10 ML
TGL 100 TRIGLYCERIDE 5 X 20 ML
TGL 200 TRIGLYCERIDE 4 X 50 ML

METHOD
Enzymatic colorimetric test

PRINCIPLE Triglycerides are determined after enzymatic hydrolysis with lipases. The quinonemine indicator is formed from hydrogen peroxide, 4-aminophenazone, and 4-chlorophenol under the catalytic influence of peroxidase.

SAMPLE
Serum. Heparinised plasma or EDTA plasma.

Stability
3 days at 2 - 8°C.

REAGENTS
Reagnet I : Enzyme regent
Reagent II : Buffer reagent
Reagent III : Triglyceride Standard : 200 mg/dl

REFERENCE VALUES
Men 60 - 165 mg/dl
Women 40 - 140 mg/dl

QUALITY CONTROL :
Accutestrol N - H

REFERENCES
Buccolo G., David M., Clin. Chem, 19 , (1973), 476

REAGENT PREPARATION
 Dissolve the contents of one vial of enzyme reagent with the volume of buffer reagent as specified on the enzyme vial.
STABILITY: The working reagent is stable for 120 days at 2-8°C. store protected from sunlight.

AUTOMATED PARAMETERS
Wave length 520 nm
Reaction Type End Point
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against reagent blank
Sample/Reagent Ratio 1: 100
Incubation 5 minutes
Maximum blank absorbance 0.50
Low Normal at 37°C 40 mg/dl
High Normal at 37°C 165 mg/dl
Linearity at 37°C 1000 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
SAMPLE - - 10 µl
STANDARD - 10 µl -
REAGENT 1000 µl 1000 µl 1000 µl

Mix well, incubate for 5 minutes at 37°C (or 15 mins at 20 - 25°C). Measure absorbance of the sample (At) and standard (As) against reagent blank. The colour is stable for 30 min at 20 - 25°C

CALCULATION AND LINEARITY
AT / AS X conc. Std. = mg/dl Triglycerides

The method is linear to a concentration of 1000 mg/dl. Dilute samples above this concentration 1 : 1 with 0.9% saline and ressay. Multily the result by 2.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com