ACCUCARETM
BUN UV
(Kinetic test UV - F.T.)
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
BUN 50 BUN UV 5 x 10 ml
BUN 100 BUN UV 5 X 20 ml
BUN 200 BUN UV 4 x 50 ml

METHOD
 Kinetic UV method

PRINCIPLE Urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia combines with alpha-oxoglutarate and NADH in the presence of glutamate-dehydrogenase to yield glutamate and NAD. The rate of decrease of NADH is directly proportional to the urea concentration

SAMPLE:
Serum or plasma, urine dilute 1:20.
Dilute urine 1+20 with distilled water. Multiply the result by 21.

Stability in the serum / plasma : 3 days at +4°C.

REFERENCE VALUES
Serum plasma 7-24 mg/dl
Urine 9.3-16.35 mg/dl

QUALITY CONTROL :
Accutestrol N - H

REAGENTS
Reagent I : Buffer reagent
Reagent II : Enzyme reagent
Reagent III :BUN Std : 20 mg/dl


REAGENT PREPARATION
Dissolve the contents of enzyme vial with the volume of buffer reagent as specified on the enzyme vial.


STABILITY: 3 months at 2-8°C 		AUTOMATED PARAMETERS
Wave length 340 nm
Measurement Against distilled water
Reaction Type Fixed time kinetic
Cuvette 1 cm light path
Reaction Temperature 37°C
Reaction direction Decreasing
Sample/Reagent Ratio 1: 100
Delay /Lag/ time 30 Secs
Interval Time 60 Secs
No: of Readings 01
Blank Absorbance Limit >0.8
Low Normal at 37°C 7-24 mg/dl
High Normal at 37°C 50 mg/dl
Linearity at 37°C 300 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  STD SAMPLE
SAMPLE - 10 µl
STANDARD 10 µl -
REAGENT 1000 µl 1000 µl

Mix well and read after 30 secs initial absorbance of the sample (A1s) and standard (A1std) and start timer simultaneously. Read again, after 60 secs.

CALCULATION AND LINEARITY
(A2s-A1s) / (A2std-A1std) x 20 = mg/dl BUN
(A2s-A1s) / (A2std-A1std) x 20 = mg/dl BUN

The method is linear to a concentration of 300 mg/dl. Dilute samples above this concentration 1+1with 0.9% Nacl solution and repeat assay. Multily the result by 2.

NOTE :
All anticoagulants except ammonia-heparinate can be used


REFERENCES
Teitz.N.W; Fundamentals of clinical chemistry,Philadelphia, W.B.Saunders & Co,. Philadelphia, p991 (1976)., Talke H. Schubert GE, Klin Wchers., (1965), 43, 174.
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