ACCUCARETM
URIC ACID
Enzymatic colorometric metric method
ORDER INFORMATION
Catalog No: Product Description: Pack Size:
URI 50 URIC ACID 5 x 10 ml
URI 100 URIC ACID 5 X 20 ml
URI 200 URIC ACID 4 x 50 ml

METHOD
 Enzymatic colorometric test

PRINCIPLE
Uric acid is converted by uricase to allantoin and hydrogen peroxide which under the catalytic influence of peroxidase, oxidizes 3, 5 - dichloro - 2 - hydroxybenzenesulfonic acid and 4-aminophenazone to form a red-violet quinoneimine compound.

REAGENTS
Reagent I : Buffer
Reagent Reagent II : Enzyme reagent
Reagent Reagent III : Uric Acid standard : 5 mg/dl

SAMPLE
Serum, heparinised or EDTA plasma, urine.
Dilute urine 1 : 10 with distilled water.
Multiply the result by 10.

Stability : 5 days at 4 - 25°C.

REFERENCE VALUES
Serum  
Men 3.4-7.0 mg/dl
Women 2.4-5.7 mg/dl
Urine 250-750 mg/24h

QUALITY CONTROL
Accutestrol N - H

REFERENCES
 Caraway, W.T., Clin Chem.4, 239 (1963), Morin L.G., Clin Chem, 20, 51 (1974) Trivedi R.C., Rebar L., Berka E., Strong L., Clin. Chem., (1978) , 24 , 1908.
 REAGENT PREPARATION
Dissolve contents of one vial of enzyme reagent with the volume of buffer reagent as specified on the enzyme vial.

STABILITY : 60 days at 2 - 8°C /. (store protected from light)

AUTOMATED PARAMETERS
Wave length 340 nm
Cuvette 1 cm light path
Reaction Temperature 37°C
Measurement Against reagent blank
Reaction Type End Point
Reaction Direction Increasing
Sample/Reagent Ratio 1: 40
Incubation 10 minutes
Maximum Blank Absorbance 0.50
Low Normal at 37°C 2.4 mg/dl
High Normal at 37°C 7.0 mg/dl
Linearity at 37°C 20 mg/dl

MANUAL PROCEDURE

PIPETTE INTO TEST TUBE
  BLANK STD SAMPLE
SAMPLE - - 10 µl
STANDARD - 10 µl -
REAGENT 1000 µl 1000 µl 1000 µl

Mix well, incubate for 10 mins at 37°C (or 15 mins at 20-25°C). Measure absorbance of sample (Ac) and standard (As) against reagent blank. The color is stable for 60 mins at 20-25°C.

CALCULATION AND LINEARITY
Ac/As x Conc of standard = mg/dl Uric Acid

This method is linear upto a concentration of 20 mg/dl.
Dilute samples above this concentration 1 : 1 with 0.9 % saline and reassay. Multiply the result by 2.
Lab Care Diagnostics (India) Pvt. Ltd.
16-A, 'A' Wing , 1st Floor Sita Estate Mahul RoadAziz Baug, Chembur Mumbai 400 074. India
Phone: 00 91 22 2554 2109/ 00 91 22 2554 1558 Fax: 00 91 22 2554 3541
E-mail : accucare@labcarediagnostics.com